Composite

Part:BBa_K1947015:Design

Designed by: Jie Tang   Group: iGEM16_NEFU_China   (2016-10-13)


The test of the Pmsp3 promoter.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 150
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 150
    Illegal NgoMIV site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1122


Design Notes

EGFP is derived from the laboratory and used as a reporter. EGFP can be expressed in prokaryotes, so we can use it in AMB-1.


Source

Pmsp3 is one of the promoters in AMB-1. EGFP is from lab data and it is used as a reporter.

References